Part:BBa_K3790000:Design

Bst polymerase I (large fragment) for E. coli

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The following primers were used to amplify Twist synthesis DNA.

Bst_LargeSec_im_CE_forward1	5-gtgccgcgcggcagccatatgGCTGAGGGCGAAAAGCCG
Bst_LargeSec_im_CE_reverse1	5-acggagctcgaattcggatccTTATTTCGCATCGTACCAGGTCG

The following primers were used for Sanger sequencing.

Bst_LargeSec_forward1	5-GCCGAAGGGGAGAAACCG
Bst_LargeSec_reverse1	5-TTATTTGGCATCATACCATGTTGGG
Bst_LargeSec_forward2	5-GCCGAAGGGGAGAAACC
Bst_LargeSec_reverse2	5-TTATTTGGCATCATACCATGTTGG